Water quality and diarrhoeagenic Escherichia coli detection in surface Pampean aquatic systems

Abstract

Many surface water systems are impacted by point source pollution from sewage discharges and industrial wastes, as well as diffuse pollution from agriculture and livestock farming, inducing a potential biohazard to human, animal, and environmental health. This study aimed to determine the presence of diarrhoeagenic Escherichia coli (DEC) pathotypes and their antibiotic resistance, as well as the bacteriological, physical, and chemical water quality conditions in two Pampean peri-urban rivers (Rojas and Salado rivers, Buenos Aires, Argentina) used for recreation. Additionally, we explored the impact of the surrounding land use on the water quality. In the Rojas (R) and Salado (S) rivers, wastewater discharges from treatment plants increased nutrient content and coliform abundances at specific sampling sites (R2 and S3) and downstream (R3 and S4, respectively). Coliform abundances correlated with ammoniacal nitrogen concentrations, both exceeding recreational use guidelines. Out of 36 samples positive for DEC virulence factors, 11 DEC strains were isolated (5 enteroaggregative, 3 enteropathogenic, 1 shigatoxigenic-stx1/stx2, 1 shigatoxigenic-stx2, 1 hybrid enteroaggregative-enterotoxigenic). Six strains were resistant to one or more antibiotics. Our results suggest that differences in E. coli pathotypes between the two rivers and the water quality of each sampling site are linked to the surrounding land use, evidencing both diffuse and point source pollution.